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Image Search Results
Journal: eLife
Article Title: Exploring conformational equilibria of a heterodimeric ABC transporter
doi: 10.7554/eLife.20236
Figure Lengend Snippet: Schematic of spin-labeled sites in the extracellular, intracellular and NBD regions of TM287/288 in ( A ) the inward-facing apo crystal structure (PDB: 4Q4H) and in ( B ) the outward-facing homology model based on the Sav1866 crystal structure (PDB: 2HYD). TM287 is colored in cyan and TM288 in pink. ( C – E ) Simulations of the distance distribution probabilities for the six spin-labeled double mutants in the IF (cyan) and OF (magenta) states represented in panels A and B. The ambient temperature MTSL rotamer library in MMM2015 was used. Comparison with the experimental data and with a previous version of the MTSL library are presented in . DOI: http://dx.doi.org/10.7554/eLife.20236.002
Article Snippet: Optimal spin-labeled pair positions were selected using the available PDB data and the rotamer library approach for the MTSL ([1-Oxyl-2,2,5,5-tetramethyl-∆3-pyrroline-3-methyl] Methanethiosulfonate) spin-labeled side chains available in the
Techniques: Labeling, Comparison
Journal: eLife
Article Title: Exploring conformational equilibria of a heterodimeric ABC transporter
doi: 10.7554/eLife.20236
Figure Lengend Snippet: Simulations of the distance distribution probability for the six spin-labeled pairs in the IF (cyan) and OF (magenta) states performed with MMM2015 with the rotamer library at ambient temperature (dotted lines as in ) and with the rotamer library of MMM2013 simulated at 175 K (dashed lines). The experimental distance distributions on nucleotide-free (apo, black solid line in upper plots) and ATP-Vi-Mg (black, solid line in lower plots) states are taken from . Transparent cyan and magenta rectangles outline the range of experimental distances characteristic of the IF and OF conformations, respectively. DOI: http://dx.doi.org/10.7554/eLife.20236.003
Article Snippet: Optimal spin-labeled pair positions were selected using the available PDB data and the rotamer library approach for the MTSL ([1-Oxyl-2,2,5,5-tetramethyl-∆3-pyrroline-3-methyl] Methanethiosulfonate) spin-labeled side chains available in the
Techniques: Labeling